|
KMID : 0358420070500030429
|
|
Korean Journal of Obstetrics and Gynecology
2007 Volume.50 No. 3 p.429 ~ p.438
|
|
|
Association of the vascular endothelial damage and estrogen, progesterone
|
|
Kim Mi-Kyoung
Kim Ji-Ae
Jeon Yeo-Jin
Park Jong-Soon
Park Mi-Hye
Suh Suk-Hyo
Chun Sun-Hee
Ahn Jung-Ja
Kim Young-Ju
|
|
|
Abstract
|
|
|
Objective: The aim of this study were to examine the serum level of estradiol, estriol, progesterone, oxidized LDL in preeclamtic patients and to evaluate the protective effects of estrogen and progesterone against lysophosphatidylcholine (LPC) induced cell death in Human umbilical vein endothelial cells (HUVECs).
Methods: We analysed the serum level of estradiol, estriol, progesterone, oxidized LDL in patients with preeclampsia and control. We used LPC to induce cell death in HUVECs. For cytotoxic assay, we did LDL assay for cell death and Resazurin assay for cell viability. HUVECs were exposed to various concentrations of LPC, LPC+estrogen, LPC+progesterone and we did cytotoxic assay.
Results: The serum estradiol, estriol were lower in the preeclamptic patients (P<0.05). Oxidized LDL were higher in the preeclamptic patients(P<0.05). LPC induced cell death in a concentration-dependant manner. Estrogen or progesterone inhibited LPC-induced cell death in a concentration-dependant manner (P<0.05).
Conclusion: Estrogen and progesterone attenuated LPC-induced cytotoxicity. The results suggest that Oxidized LDL induced endothelial damage in preeclampsia may be induced by low serum estradiol, estriol and progesterone levels and prevented by estrogen and progesterone addition.
|
|
|
KEYWORD
|
|
|
Endothelial damage, Estrogen, Progesterone
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
  
|
|